美***C.B.S.SCIENTIFIC脂蛋白凝膠電泳Pore Gradie nt Gel Electr
Resolve Lipoprotein Sub-Classes
APPLICATIONS
Resolution of Lipoprotein subclasses (HDL and LDL)
Standard PAGE
DESCRIPTION
The Pore Gradient Lipoprotein Electrophoresis System (LPE) is designed to
resolve lipoprotein sub-classes of low density lipoproteins (LDL) and high
density lipoproteins (HDL) based on particle size using non-denaturing pore
gradient gel electrophoresis (1, 2, 3). Other applications include standard
PAGE and short DNA sequencing runs. Temperature control (8°C - 10°C) is
accomplished using a cooling coil in the buffer chamber that is connected
to an external refrigerated water bath. A built-in pump recirculates the buffer
and helps keep the temperature constant.
CBS Scientific has specifically designed this system to be used with pre-cast
non-denaturing acrylamide gradients from Alamo Gels, Inc. Up to four gels
can be run simultaneously. Slots in a specially molded silicone gasket hold
these pre-cast gels in place in the upper reservoir. 3 gel slot plugs are included
in the event the researcher would like to run less than 4 gels at a time.
Precast Non-denaturing Pore-Gradient Polyacrylamide Gels
C.B.S. Scientific offers 2/16, 4/30 and 2/31 percent pre-cast non-denaturing
polyacrylamide gels for high resolution gradient gel electrophoresis. The 2/16
gels are for resolving low-density lipoproteins (LDL’s) over an approximate
molecular weight range of 100,000-5,000,000. The 4/30 gels are for
resolving high-density lipoproteins (HDL’s) over an approximate range of
50,000-2,000,000. The 2/31 combo gels resolve lipoproteins from 50,000-
5,000,000. The pre-cast cassettes are 4.9mm thick with a height and width
of 82mm. Gels are sold in packs of 16, have a 6 month shelf life and should
be kept at 4°C - 8°C.
Glass Plates and Spacers
Glass plates and spacers can be ordered for researchers that desire to pour
their own gradient gels. Glass plates dimensions are 8.2cm wide by 8.2cm
high (cat. #LPG-082). Spacers are 82mm high and have a thicknesses of
2.8mm (cat. #LPS-028).
Sample Applicators
Sample applicators can be ordered with a choice of 12 (cat. #LPE-SL12),
13 (cat. #LPE-SL13), or 18 (cat. #LPE-SL18) wells. Sample applicators are
inserted into the top of the pre-cast gels for sample loading and remain in
place during the electrophoresis.
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